Benefits of working with us

Real Research team is ready and open to work with you

  • 3D cell culture customization
  • analysis of your project to meet your needs and expectations
  • direct consultation with experienced chemists and biotechnologists
  • responsible and flexible team
  • technical and technological knowledge
  • experience in solving difficult analytical projects

 The difficult we do immediately. The impossible takes a little longer.

Our body is 3D so the research should be too!

Real Research provides the LifeGel for your experiments and can also optimize the LifeGel for a specific cell type or experiment of your choice. If you need a customised service please contact us. 

Comparison of 2D vs. 3D cell culture

Long-term 3D cultures

IC50  (measurement of the potency of a substance)

In vitro tube formation assay, Aortic ring assay

Intestinal permeability assay

Comparison of 2D vs. 3D cell culture

Figure 1. Pancreatic cancer cell line cultured in 2D conditions

Figure 2. Pancreatic cancer cell line cultured in 3D conditions

Even though the two-dimensional (2D) cell culture on a standard cell culture plastic dish gives very doubtful results, it is still the most widely used cell culture method.

By a comparison of 2D vs. 3D cell culture you can verify if your standard 2D cell culture gives the same results in a much more physiological 3D cell culture microenvironment.  

Long-term 3D cultures

4T1 cell line after 14 days cultured on LifGel

4T1 cell line after 17 days cultured on LifGel

4T1 cell line after 20 days cultured on LifGel

Long-term cultures enable you to see how your cells behave during prolonged growth in 3D conditions. This assay comes in handy while testing different drug concentrations/time periods and it is also an excellent tool for obtaining cells that are sensitive or resistant to a specific compound.

In vitro tube formation assay, Aortic ring assay

Angiogenesis is a process of new blood vessels formation, taking a key role in certain physiological conditions such as wound healing and function of female reproducible organs. An assay used to determine the ability of endothelial cells to create capillary-like structures in the desired condition. An excellent tool for examining the effect of studied compounds on tube formation, it is often used in oncology as it is very useful for testing drugs that affect tumor angiogenesis.

An aortic ring assay is a useful tool for examining in vitro angiogenesis using aortic tissue samples, which is impossible on most commercially available 3D platforms.

Figure 3. HUVEC cell line on LifeGel, in vitro tube formation assay

Intestinal permeability assay

Figure 3. Caffeine absorption – Colorectal adenocarcinoma cell line cultured on LifeGel (Papp- permeability coefficient)

The small and large intestines function are a digestive/absorption organ for nutrients, as well as a strong immunological and mechanical barrier preventing the excessive absorption of bacteria, food antigens and other macromolecules.

Analysis of the absorption and bioavailability of a given substance in the intestine is especially useful for testing drugs intended for oral consumption in order to determine the adsorption kinetics.


(+48) 603-984-577


Real Research sp. z o.o.
Jagiellonian Center of Innovation
prof. M. Bobrzyńskiego 14
30-348 Kraków, Poland


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