LifeGel for cancer spheroid formation


LifeGel for 3D cell culture is the product consisting of multi-well plate covered by the LifeGel in the presence of a cell culture medium or PBS. There are two mains options of this product: standard and customized.

To learn more about type of LifeGels go to OPTIONS.


LifeGel technology gives the possibility to recreate the cells natural microenvironment

LifeGel is not a single hydrogel for three-dimensional (3D) cell culture - it is a technology platform.

Specification of LifeGel for 3D cell culture
Ready to use hydrogel on a standard multi-well plate for cell culture in a PBS or cell culture medium
The hydrogel is colorless
48-well plate with 250 μL of LifeGel per well or 96-well plate with 120 μL of LifeGel per well
6 months
Store at 4°C

Product is intended for research use only, not for diagnostic or therapeutic purposes.

Batch to batch repeatability

Techniques applicable for use with LifeGel

1. Imaging

2. Long term cell culture – e.g. three weeks 

3. Easy cell harvesting due to simple to use enzymatic LifeGel Digestion Kit
for: cell passaging, cell counting or any other experiments
where these cell will be necessary 

4. Cell imaging with equipment like for example: JULI Stage, Celloger
or Opera Phoenix 

5. Readout your assay on standard plate reader
for example measure viability with Cell Titer Blue or Cell Titer Glo 3D 

6. IHC (Immunohistochemistry)

7. Compatible with standard automatic pipetting equipment

No prior preparation – just ready to use plates!





LifeGel technology gives the possibility to match the perfect hydrogel for each cell line. LifeGel technology is a panel of hydrogels with a range of elasticity from 180 Pa to 5 kPa.

Based on our many years of experience we have selected one type of the hydrogel that is suitable for most of standard 3D cell culture – STANDARD TYPE of LifeGel for 3D cell culture. 

We know that carrying out a sophisticated research is related with a very carefully selecting of research model. To meet the expectations we offer the CUSTOMISED TYPE of LifeGel for 3D cell culture. If you are interested in the CUSTOMISED TYPE please contact us or you can choose your type of LifeGel testing the product Panel of LifeGelsIf you have already tested the Panel of LifeGels and you know which type of LifeGel is the best for your research, select the type CUSTOMISED and remember to inform us about your choice in the comments to the order.

All LifeGels are available in standard 48 and 96-well plate. If you need a customized format please contact us.

All LifeGels are available in standard cell culture medium: RPMI, DMEM-HG (Standard), DMEM-LG, DMEM/F-12, alpha-MEM or in PBS. If you use a different cell culture medium we recommend buying the version with PBS and incubating the hydrogel with a right medium 2 hours before starting a cell culture. If you want to buy the LifeGel in a customized cell culture medium please contact us.




(+48) 603-984-577


Real Research sp. z o.o.
Jagiellonian Center of Innovation
prof. M. Bobrzyńskiego 14
30-348 Kraków, Poland


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